The physiological parameters, training characteristics and race performance in highly trained cyclists. A cross-sectional study
DOI:
https://doi.org/10.47197/retos.v79.118526Keywords:
Amateur cyclists, maximal fat oxidation, peak power output, road cycling, VO2maxAbstract
Introduction: Physiological parameters such as maximum oxygen uptake (VO2max), Absolute and Relative Peak Power Output (PPO, PPO·kg-1) and Maximal Fat Oxidation (MFO) are critical parameters in cycling performance and race success.
Objective: This study was designed to explore these physiological parameters and training characteristics of Under 23 (U23), Elite and Master 30 (M30) cyclists and its association with races’ performance.
Method: A cross-sectional study was conducted with thirty-four highly trained road cyclists (28.3 ± 7.4 years, 177.4 ± 6.0 cm, 68.4 ± 7.3 kg) who performed a VO2max test at the beginning of the pre-season period. Linear regressions were performed to examine the association between physiological parameters and weekly training hours. Analysis of Covariance (ANCOVA) was used to compare each physiological parameter across competitive categories while adjusting for weekly training hours as a covariate. Statistical significance was set at p < 0.05.
Results: A positive association was observed between training hours and VO2max, PPO and PPO·kg-1 (all p < 0.05). No association was found between training hours and MFO. Significant differences were found between categories in terms of VO2max, PPO and PPO·kg-1 (all p < 0.05) where Elite and U23 cyclists overperformed M30 cyclists, while no significant differences were found in MFO. No significant associations were observed between race performance and the physiological parameters mentioned.
Conclusion: Training volume in highly trained cyclists appears to be a key determinant of aerobic performance in VO2max, PPO and PPO·kg-1. Elite and U23 cyclists showed higher VO2max and PPO·kg-1 values than M30 cyclists, likely due to their training status and races demands.
References
Alejo, L. B., Montalvo-Pérez, A., Valenzuela, P. L., Revuelta, C., Ozcoidi, L. M., De La Calle, V., Mateo-March, M., Lucia, A., Santalla, A., & Barranco-Gil, D. (2022). Comparative analysis of endurance, strength and body composition indicators in professional, under-23 and junior cyclists. Fron-tiers in Physiology, 13, 945552. https://doi.org/10.3389/fphys.2022.945552
Allen, H., & Coggan, A. (2010). Training and racing with a powermeter (2nd ed.). Velopress.
Burke, L. M., Hawley, J. A., Wong, S. H. S., & Jeukendrup, A. E. (2011). Carbohydrates for training and competition. Journal of Sports Sciences, 29(sup1), S17–S27. https://doi.org/10.1080/02640414.2011.585473
Cano-Giraldo, S., Ascuntar-Viteri, J. D., Quintero, J. D., Rodríguez-Hernández, A. F., Cadavid, A. M., López-Betancourt, S., Acosta-Arroyave, N., & Rojas-Jaramillo, A. (2025). Physiological profile of elite female road cyclists from Antioquia, Colombia: a cross-sectional study. Retos, 72, 589-597. https://doi.org/10.47197/retos.v72.116571
Cove, B., Chalmers, S., Nelson, M. J., Anderson, M., & Bennett, H. (2025). The effect of training distribu-tion, duration, and volume on VO2max and performance in trained cyclists: A systematic re-view, multilevel meta-analysis, and multivariate meta-regression. Journal of Science and Medi-cine in Sport, 28(5), 423–434. https://doi.org/10.1016/j.jsams.2024.12.005
Denham, J., Scott-Hamilton, J., Hagstrom, A. D., & Gray, A. J. (2020). Cycling Power Outputs Predict Functional Threshold Power and Maximum Oxygen Uptake. Journal of Strength and Condition-ing Research, 34(12), 3489–3497. https://doi.org/10.1519/JSC.0000000000002253
Fernández-Garcia, B., Pérez-Landaluce, J., Rodríguez-Alonso, M., & Terrados, N. (2000). Intensity of exercise during road race pro-cycling competition. Medicine & Science in Sports & Exercise, 32(5). https://journals.lww.com/acsm-msse/fulltext/2000/05000/intensity_of_exercise_during_road_race_pro_cycling.19.aspx
Frandsen, J., Amaro-Gahete, F. J., Landgrebe, A., Dela, F., Ruiz, J. R., Helge, J. W., & Larsen, S. (2021). The influence of age, sex and cardiorespiratory fitness on maximal fat oxidation rate. Applied Physi-ology, Nutrition, and Metabolism, 46(10), 1241–1247. https://doi.org/10.1139/apnm-2021-0080
Frayn, K. N. (1983). Calculation of substrate oxidation rates in vivo from gaseous exchange. Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology, 55 2, 628–634.
Gallo, G., Leo, P., Mateo-March, M., Giorgi, A., Faelli, E., Ruggeri, P., Mujika, I., & Filipas, L. (2022). Differ-ences in Training Characteristics Between Junior, Under 23 and Professional Cyclists. Interna-tional Journal of Sports Medicine, 43(14), 1183–1189. https://doi.org/10.1055/a-1847-5414
Gallo, G., Mateo‐March, M., Gotti, D., Faelli, E., Ruggeri, P., Codella, R., & Filipas, L. (2022). How do world class top 5 Giro d’Italia finishers train? A qualitative multiple case study. Scandinavian Journal of Medicine & Science in Sports, 32(12), 1738–1746. https://doi.org/10.1111/sms.14201
Gallo, G., Mateo-March, M., Gotti, D., Maunder, E., Codella, R., Ruggeri, P., Faelli, E., & Filipas, L. (2023). The Weekly Periodization of Top 5 Tour de France General Classification Finishers: A Multiple Case Study. International Journal of Sports Physiology and Performance, 18(11), 1313–1320. https://doi.org/10.1123/ijspp.2023-0142
Hawley, J. A., & Noakes, T. D. (1992). Peak power output predicts maximal oxygen uptake and perfor-mance time in trained cyclists. European Journal of Applied Physiology and Occupational Phys-iology, 65(1), 79–83. https://doi.org/10.1007/BF01466278
Hopkins, W. G., Marshall, S. W., Batterham, A. M., & Hanin, J. (2009). Progressive Statistics for Studies in Sports Medicine and Exercise Science. Medicine & Science in Sports & Exercise, 41(1), 3–12. https://doi.org/10.1249/MSS.0b013e31818cb278
Hovorka, M., Leo, P., Simon, D., Rumpl, C., & Nimmerichter, A. (2023). Physiological Characteristics of Competitive Male Junior Cyclists Transitioning to the Under-23 Level: A Retrospective Compar-ative Study. International Journal of Sports Physiology and Performance, 18(8), 874–877. https://doi.org/10.1123/ijspp.2022-0496
Impellizzeri, F. M., Marcora, S. M., Rampinini, E., Mognoni, P., & Sassi, A. (2005). Correlations between physiological variables and performance in high level cross country off road cyclists. British Journal of Sports Medicine, 39(10), 747–751. https://doi.org/10.1136/bjsm.2004.017236
Jamnick, N. A., Botella, J., Pyne, D. B., & Bishop, D. J. (2018). Manipulating graded exercise test variables affects the validity of the lactate threshold and V˙O2peak. PLOS ONE, 13(7), e0199794. https://doi.org/10.1371/journal.pone.0199794
Jeukendrup, A., & Achten, J. (2001). Fatmax: A new concept to optimize fat oxidation during exercise? European Journal of Sport Science, 1(5), 1–5. https://doi.org/10.1080/17461390100071507
Leo, P., Simon, D., Hovorka, M., Lawley, J., & Mujika, I. (2022). Elite versus non-elite cyclist – Stepping up to the international/elite ranks from U23 cycling. Journal of Sports Sciences, 40(16), 1874–1884. https://doi.org/10.1080/02640414.2022.2117394
Lucía, A., Hoyos, J., & Chicharro, J. L. (2001). Physiology of Professional Road Cycling. Sports Medicine, 31(5), 325–337. https://doi.org/10.2165/00007256-200131050-00004
Lucía, A. P., J; Durántez, A; Hoyos, J; Chicharro, J. L. (1998). Physiological Differences Between Profes-sional and Elite Road Cyclists. International Journal of Sports Medicine, 19(05), 342–348. https://doi.org/10.1055/s-2007-971928
Marquet, L.-A., Brisswalter, J., Louis, J., Tiollier, E., Burke, L. M., Hawley, J. A., & Hausswirth, C. (2016). Enhanced Endurance Performance by Periodization of Carbohydrate Intake: “Sleep Low” Strategy. Medicine & Science in Sports & Exercise, 48(4), 663–672. https://doi.org/10.1249/MSS.0000000000000823
Maunder, E., Plews, D. J., & Kilding, A. E. (2018). Contextualising Maximal Fat Oxidation During Exercise: Determinants and Normative Values. Frontiers in Physiology, 9, 599. https://doi.org/10.3389/fphys.2018.00599
Menaspà, P., Rampinini, E., Bosio, A., Carlomagno, D., Riggio, M., & Sassi, A. (2012). Physiological and anthropometric characteristics of junior cyclists of different specialties and performance lev-els. Scandinavian Journal of Medicine & Science in Sports, 22(3), 392–398. https://doi.org/10.1111/j.1600-0838.2010.01168.x
Midgley, A. W., McNaughton, L. R., & Carroll, S. (2006). Verification phase as a useful tool in the deter-mination of the maximal oxygen uptake of distance runners. Applied Physiology, Nutrition, and Metabolism, 31(5), 541–548. https://doi.org/10.1139/h06-023
Mirizio, G. G., Muñoz, R., Muñoz, L., Ahumada, F., & Del Coso, J. (2021). Race Performance Prediction from the Physiological Profile in National Level Youth Cross-Country Cyclists. International Journal of Environmental Research and Public Health, 18(11), 5535. https://doi.org/10.3390/ijerph18115535
Mittendorfer, B., & Klein, S. (2001). Effect of Aging on Glucose and Lipid Metabolism during Endurance Exercise. International Journal of Sport Nutrition and Exercise Metabolism, 11(s1), S86–S91. https://doi.org/10.1123/ijsnem.11.s1.s86
Mujika, I., & Padilla, S. (2001). Physiological and Performance Characteristics of Male Professional Road Cyclists: Sports Medicine, 31(7), 479–487. https://doi.org/10.2165/00007256-200131070-00003
Nikolaïdis, P. T., & Papadopoulos, V. E. (2011). Cardiorespiratory Power and Force-Velocity Character-istics in Road Cycling: The Effect of Aging and Underlying Physiological Mechanisms. Medicina Sportiva, 15(2), 68–74. https://doi.org/10.2478/v10036-011-0012-2
Peiffer, J. J., Abbiss, C. R., Chapman, D., Laursen, P. B., & Parker, D. L. (2008). Physiological Characteris-tics of Masters-Level Cyclists. Journal of Strength and Conditioning Research, 22(5), 1434–1440. https://doi.org/10.1519/JSC.0b013e318181a0d2
Pérez-Landaluce, J., Fernández-Garcia, B., Rodríguez-Alonso, M., García-Herrero, F., García-Zapico, P., Patterson, A. M., & Terrados, N. (2002). Physiological differences and rating of perceived exer-tion (RPE) in professional, amateur and young cyclists. The Journal of Sports Medicine and Physical Fitness, 42(4), 389–395.
Psilander, N., Frank, P., Flockhart, M., & Sahlin, K. (2013). Exercise with low glycogen increases PGC-1α gene expression in human skeletal muscle. European Journal of Applied Physiology, 113(4), 951–963. https://doi.org/10.1007/s00421-012-2504-8
Purdom, T., Kravitz, L., Dokladny, K., & Mermier, C. (2018). Understanding the factors that effect max-imal fat oxidation. Journal of the International Society of Sports Nutrition, 15(1), 3. https://doi.org/10.1186/s12970-018-0207-1
RFEC. (2025). Pruebas en Carretera (Updated 9 September 2025). https://yosoyciclista.s3.amazonaws.com/documentos/smartweb/menu/123/doc_68df90fc30bad7_38959529_2_Pruebas_Carretera_ap_CD_20251002_IZDA.pdf
Sallet, P., Mathieu, R., Fenech, G., & Baverel, G. (2006). Physiological differences of elite and professional road cyclists related to competition level and rider specialization. Journal of Sports Medicine and Physical Fitness, 46(3), 361–365.
San-Millán, I., & Brooks, G. A. (2018). Assessment of Metabolic Flexibility by Means of Measuring Blood Lactate, Fat, and Carbohydrate Oxidation Responses to Exercise in Professional Endurance Ath-letes and Less-Fit Individuals. Sports Medicine, 48(2), 467–479. https://doi.org/10.1007/s40279-017-0751-x
Sial, S., Coggan, A. R., Hickner, R. C., & Klein, S. (1998). Training-induced alterations in fat and carbohy-drate metabolism during exercise in elderly subjects. American Journal of Physiology-Endocrinology and Metabolism, 274(5), E785–E790. https://doi.org/10.1152/ajpendo.1998.274.5.E785
Sitko, S., Cirer-Sastre, R., & López-Laval, I. (2022). Time to exhaustion at estimated functional threshold power in road cyclists of different performance levels. Journal of Science and Medicine in Sport, 25(9), 783–786. https://doi.org/10.1016/j.jsams.2022.06.007
Toth, M., & Tchernof, A. (2000). Lipid metabolism in the elderly. European Journal of Clinical Nutrition, 54(3), S121–S125. https://doi.org/10.1038/sj.ejcn.1601033
Valenzuela, P. L., Muriel, X., Van Erp, T., Mateo-March, M., Gandia-Soriano, A., Zabala, M., Lamberts, R. P., Lucia, A., Barranco-Gil, D., & Pallarés, J. G. (2022). The Record Power Profile of Male Profes-sional Cyclists: Normative Values Obtained From a Large Database. International Journal of Sports Physiology and Performance, 17(5), 701–710. https://doi.org/10.1123/ijspp.2021-0263
Van Erp, T., Lamberts, R. P., & Sanders, D. (2022). Power Profile of Top 5 Results in World Tour Cycling Races. International Journal of Sports Physiology and Performance, 17(2), 203–209. https://doi.org/10.1123/ijspp.2021-0081
Van Erp, T., & Sanders, D. (2021). Demands of professional cycling races: Influence of race category and result. European Journal of Sport Science, 21(5), 666–677. https://doi.org/10.1080/17461391.2020.1788651
Downloads
Published
Issue
Section
License
Copyright (c) 2026 David Gómez-Cazorla, Raúl López-Grueso, Jordi Vicens-Bordas

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and ensure the magazine the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of authorship of the work and the initial publication in this magazine.
- Authors can establish separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Is allowed and authors are encouraged to disseminate their work electronically (eg, in institutional repositories or on their own website) prior to and during the submission process, as it can lead to productive exchanges, as well as to a subpoena more Early and more of published work (See The Effect of Open Access) (in English).
This journal provides immediate open access to its content (BOAI, http://legacy.earlham.edu/~peters/fos/boaifaq.htm#openaccess) on the principle that making research freely available to the public supports a greater global exchange of knowledge. The authors may download the papers from the journal website, or will be provided with the PDF version of the article via e-mail.